Plasmid Purification-Kits I-Blue Mini / Midi
For rapid and efficient extraction of plasmid DNA from bacterial cells.
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.
-Fast extraction time
-High yield
-Prevention of handling errors during cell lysis and neutralisation by I-Blue colour indicator
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.
-Fast extraction time
-High yield
-Prevention of handling errors during cell lysis and neutralisation by I-Blue colour indicator
| Specifications | I-Blue Mini Plasmid Kit // I-Blue Midi Plasmid Kit |
| Method: | Spin column // Anion-Exchange |
| Sample size: | 1 - 7 ml // 50 - 100 ml high-copy plasmid / 100 - 150 ml low-copy plasmid |
| Binding capacity: | 50 µg // 500 µg |
| Fragment size: | 1 - 15 kb // 1 - 20 kb |
| Typical yield: | up to 50 µg // 200 - 500 µg |
| Operation Time: | <15 min. // <80 min. |
TypeNumber of
test fieldsVolume sampleFragment sizeYield
test fieldsVolume sampleFragment sizeYield
µg
Binding capacityµg
Product specific notesPreparation timemin
PKPrice/UnitEUR

μg
50μg
Spin column15min
128,80€
34,27€
incl. Tax Add tobasket

μg
50μg
Spin column15min
1209,65€
249,48€
incl. Tax Add tobasket

μg
50μg
Spin column15min
1579,70€
689,84€
incl. Tax Add tobasket

μg
500μg
Anion-Exchange, gravity flow80min
1308,50€
367,12€
incl. Tax Add tobasket

TypeI-Blue MINI
Number of
test fields4
Volume sample1 ... 7 ml
Fragment size1 ... 15 kb
Yield
µg
<50μg
Binding capacity
µg
50μg
Product specific notesSpin column
Preparation time
min
15min
PK1
1 VE28,80
€
EUR34,27€
EUR incl. Taxplease selectAdd to
basket
basket

TypeI-Blue MINI
Number of
test fields100
Volume sample1 ... 7 ml
Fragment size1 ... 15 kb
Yield
µg
<50μg
Binding capacity
µg
50μg
Product specific notesSpin column
Preparation time
min
15min
PK1
1 VE209,65
€
EUR249,48€
EUR incl. Taxplease selectAdd to
basket
basket

TypeI-Blue MINI
Number of
test fields300
Volume sample1 ... 7 ml
Fragment size1 ... 15 kb
Yield
µg
<50μg
Binding capacity
µg
50μg
Product specific notesSpin column
Preparation time
min
15min
PK1
1 VE579,70
€
EUR689,84€
EUR incl. Taxplease selectAdd to
basket
basket

TypeI-Blue MIDI
Number of
test fields25
Volume sample50 ... 150 ml
Fragment size1 ... 20 kb
Yield
µg
200 ... 500μg
Binding capacity
µg
500μg
Product specific notesAnion-Exchange, gravity flow
Preparation time
min
80min
PK1
1 VE308,50
€
EUR367,12€
EUR incl. Taxplease selectAdd to
basket
basket