CHROMABOND^® Easy / Macherey-Nagel

Polar modified polystyrene-divinylbenzene copolymer with a weak anion exchanger

Specific surface 650 to 700 m²/g, particle size 80µm, pore size 50 Å, pH stability 1 to 14. Due to bifunctional modification much more hydrophilic than conventional polystyrene-divinylbenzene polymers and thus easily wettable with water.

Recommended applications: polar herbicides / pesticides from water (acidic, neutral, basic), polar phenols from water, polyaromatic compounds, polychlorinated biphenyls, drug analysis from urine, blood, serum, plasma pharmaceuticals / active ingredients from tablets, creams.

Due to the bifunctional modification CHROMABOND^® Easy is considerably more hydrophilic than conventional polystyrene-divinylbenzene polymers and thus easily wettable with water. The Easy effect: Aqueous samples can be loaded directly without preconditioning! This means that little or even no conditioning is needed, in contrast to standard SPE materials, where recovery rates normally decrease, in the worst case down to zero! Depending on the separation task conditioning may be required and is recommended for method development. A positive side effect of the excellent wettability: there is no decrease of recovery rates, if the cartridge runs dry, therefore automation is easier or, in some cases compared to silica materials, only feasible with CHROMABOND^® Easy, because a permanent vacuum can be used without supervision.

Further advantages of using a polymeric material:
-high surface, this means very high binding capacity (2 - 5 times higher than silica-based adsorbents)
-less adsorbent is needed in the cartridge (without losing sensitivity or recovery)
-faster analysis, because the height of the adsorbent bed can be reduced
-acidic or basic solvents (e. g. TFA) do not destroy the phase, or lead to unintended side products
Because of the polar modification the material is suitable for a broad range of compounds (acidic, neutral, basic, polar and nonpolar substances). Highly reproducible recovery rates can be obtained, even if the cartridge runs dry (especially advantageous when using 96-well plates, where stopcocks are not available!)